| 质粒名称: | pX330 p53 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 59910 |
| 存储实验室: | Tyler Jacks |
|
载体骨架基本信息 |
|
| 载体名称: | pX330 |
| 载体出品公司: | Feng Zhang lab (Addgene plasmid # 42230) |
| 空载体大小: | 8500 bp |
| 完整质粒大小: | 8520 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,CRISPR |
| 筛选标记: | - |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | sgRNA targeting p53 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | U6 |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BbsI（克隆期间销毁） |
| 3’克隆位点: | BbsI（克隆期间销毁） |
| 5’测序引物 | hU6-F |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pX330 p53 was a gift from Tyler Jacks (Addgene plasmid # 59910 ; http://n2t.net/addgene:59910 ; RRID:Addgene_59910) |
| 参考文献部分: | CRISPR-mediated direct mutation of cancer genes in the mouse liver. Xue W, Chen S, Yin H, Tammela T, Papagiannakopoulos T, Joshi NS, Cai W, Yang G, Bronson R, Crowley DG, Zhang F, Anderson DG, Sharp PA, Jacks T. Nature. 2014 Aug 6. doi: 10.1038/nature13589. 10.1038/nature13589 PubMed 25119044 |
买家导航
pX330p53载体基本信息
pX330p53载体图谱质粒图谱和多克隆位点信息
pX330p53载体简介
pX330p53载体序列
LOCUS pX330 p53 8487 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION pX330 backbone expressing sgRNA targeting p53 to edit mouse p53.
Expresses Cas9 from CBh promoter.
ACCESSION .
VERSION .
KEYWORDS pX330 p53
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8487)
AUTHORS Xue W, Chen S, Yin H, Tammela T, Papagiannakopoulos T, Joshi NS, Cai
W, Yang G, Bronson R, Crowley DG, Zhang F, Anderson DG, Sharp PA,
Jacks T
TITLE CRISPR-mediated direct mutation of cancer genes in the mouse liver.
JOURNAL Nature. 2014 Aug 6. doi: 10.1038/nature13589.
PUBMED 25119044
REFERENCE 2 (bases 1 to 8487)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8487
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..241
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 1..21
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 172..191
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 271..346
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
enhancer 443..728
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 730..986
/label=chicken beta-actin promoter
/note="contains a 21-bp deletion relative to the standard
chicken beta-actin promoter"
intron 987..1214
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
regulatory 1226..1235
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1235..1300
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 1307..1327
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1352..5452
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5453..5500
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
primer_bind complement(5528..5545)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5534..5741
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 5750..5890
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 5750..5879
/label=AAV2 ITR
rep_origin 5965..6420
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6052..6071)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6262..6283
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6437..6456)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6556..6578
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6616..6634)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6702..6806
/gene="bla"
/label=AmpR promoter
CDS 6807..7667
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7025..7044)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7838..8426
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8327..8346
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
241 cgaaacaccg cctcgagctc cctctgagcc gttttagagc tagaaatagc aagttaaaat
301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga
361 gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca
421 gacaaatggc tctagaggta cccgttacat aacttacggt aaatggcccg cctggctgac
481 cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac
541 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
601 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc
661 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
721 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
781 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
841 gggggggggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca
901 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
961 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc
1021 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
1081 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gctgagcaag aggtaagggt
1141 ttaagggatg gttggttggt ggggtattaa tgtttaatta cctggagcac ctgcctgaaa
1201 tcactttttt tcaggttgga ccggtgccac catggactat aaggaccacg acggagacta
1261 caaggatcat gatattgatt acaaagacga tgacgataag atggccccaa agaagaagcg
1321 gaaggtcggt atccacggag tcccagcagc cgacaagaag tacagcatcg gcctggacat
1381 cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa
1441 attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct
1501 gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag
1561 atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc
1621 caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa
1681 gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa
1741 gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct
1801 gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga
1861 gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac
1921 ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat
1981 cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg
2041 cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc tgacccccaa
2101 cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta
2161 cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct
2221 ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga
2281 gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga
2341 cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca aagagatttt
2401 cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca gccaggaaga
2461 gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg aactgctcgt
2521 gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc
2581 ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag atttttaccc
2641 attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca tcccctacta
2701 cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa agagcgagga
2761 aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg cccagagctt
2821 catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc tgcccaagca
2881 cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga aatacgtgac
2941 cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct
3001 gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa
3061 aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct
3121 gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga
3181 aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat
3241 gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct
3301 gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg
3361 ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag
3421 aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc
3481 ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc
3541 cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat
3601 gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca
3661 gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct
3721 gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct
3781 gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa
3841 ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga aggacgactc
3901 catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc
3961 ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct
4021 gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact
4081 ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt
4141 ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg
4201 ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca
4261 gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc
4321 cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg
4381 cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa
4441 ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac
4501 cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga
4561 gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca
4621 agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct
4681 gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta
4741 cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa
4801 gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag
4861 aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa
4921 aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa
4981 gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa
5041 atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga
5101 taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga
5161 gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct
5221 gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca
5281 cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat
5341 cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag
5401 catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg acaaaaggcc
5461 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag taagaattcc tagagctcgc
5521 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
5581 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
5641 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
5701 aagggggagg attgggaaga gaatagcagg catgctgggg agcggccgca ggaaccccta
5761 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
5821 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
5881 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
5941 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
6001 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
6061 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
6121 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
6181 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
6241 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
6301 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
6361 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
6421 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
6481 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
6541 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
6601 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
6661 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
6721 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
6781 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
6841 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
6901 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
6961 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
7021 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
7081 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
7141 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
7201 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
7261 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
7321 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
7381 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
7441 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
7501 aatctggagc cggtgagcgt ggaagccgcg gtatcattgc agcactgggg ccagatggta
7561 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
7621 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
7681 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
7741 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
7801 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
7861 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
7921 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
7981 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
8041 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
8101 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
8161 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
8221 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
8281 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
8341 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
8401 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
8461 ccttttgctg gccttttgct cacatgt
//
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